Native Rtas
Capparis spinosa is a shrub Capparidaceae family interest, and is a native of Israel
and in eastern part of the Mediterranean. Have little long and thorny branches, Round
leaves. The flower is big, white, 4 petals and has a lot of time, pink stamens. The bush is full of
hunters for a long period. The Capar had other uses in the kitchen. The first documented use of the
Capable Bush was for medicinal purposes in 2000 a. C., by the Sumerians. The ancient Greeks and
Romans also used the plants for these purposes. Under conditions of Hungary could be a
exotic potted plant or a plant cube. The conventional seed propagation is deserted and slow,
because the germination percentage is very low. This problem can be solved by the in vitro
propagation. Our goal was to develop the method of in vitro propagation (micropropagation) of
Capparis. The Cultuie started from seeds that were collected in Israel. The first step
Clorox surface sterilized seeds were soaked in 50% solution Clorox (100 ml 100 ml
sterile distilled water) for hours of the UN. After stirring the solution with the seeds of an independent film
seeds. After the seeds are sterilized with 0.1% HgCl2 for 5 minutes and washed in
Destillates sterile water 3 times. The seeds were placed in the middle of S3 for germination. Half of the
seeds are put in the refrigerator for 8 months to 5 ° C, and the other part was germinated in
normal crop condition were incubated at 18 -25 ° C in 8 / 16 hours dark / light and CuCl
illuminated with fluorescent lamps with an intensity of 40? M / m / s. for germination and
s basal proliferation (through macro-BM elements: Ja'mbor-Benczu'ry Ma'rta,
1990, Heller, 1953 micro-elements and vitamins MS: Murashige and Skoog, 1962) was used.
The basal medium was supplemented for the germination of 3 mg / 1 2iP (S3) and the proliferation of 1-5
mg / 1 kinetin 20 g / 1 sucrose and 7.5 g / 1 agar (C1-C5). The pH was adjusted to 5.6 in
each case using KOH. The media were sterilized at 10 Pa pressure of 35 minutes. The percentage
germination in vitro was 6 weeks. The germination of the seeds were placed in the middle of C1 first to grow
until the seedlings were reduced node pieces and pass a means of proliferation C2.The
seedlings and propagules to maintain its original number, the new plants are planted
clones originated. Plants of origin in 1,2,4,6,10 and 11 clones of seeds were placed on C4
means for comparing the ability to spread. The culture period was 6 mounts. The best
results were achieved by the two points 11, the average number of outbreaks was 14.8. It was followed
by the colon 2 (12.1) and 10 (8.4 shoots). The two points 1 and 6 only average 5.8 and 5.8.
result means that the spread ratio depends on two points (genotypes). For the next
experiment colon 10 was used, and the buds were placed in different media: basal medium S
, C1 – C5 media. In the S support is not spread, but outbreaks have roots in 6.7
average. In support of C1 1.5 shoots have roots 9.6. There were no roots in the C2 C3 and
Middle C4, the number of outbreaks was 2.6,4.1 and 5.1 after 6 weeks of culture. In the half C5
only a few outbreaks were found, but its shape was abnormal. It can be concluded that C1
best way to eradicate then medium S. For the proliferation of the medium proved the best.
About the Author:
Horshati E. The Hebrew University (1) Benczur E. Budapest University (2)
Article Source: ArticlesBase.com – IN VITRO PROPAGATION OF CAPPARIS SPINOSA
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